Application

Research Sub CategoryNuclear Receptors

This Anti-DNA polymerase II subunit A Antibody, clone 9F11.1 is validated for use in Western Blotting for the detection of DNA polymerase II subunit A .

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7; UniProt Q07864; also known as DNA polymerase II subunit A) is encoded by the POLE (also known as CRCS12, FILS, POLE1) gene (Gene ID 5426) in human. DNA polymerase II is one of the six known mammalian template-directed DNA polymerases. It is composed of two subunits (261 kDa and 59 kDa). The larger catalytic subunit is a B-type polymerase family member, containing polymerase and intrinsic 3′-5′ exonuclease activities in its N-terminal part and a putative zinc-finger domain in its C-terminus. POLE gene mutations are linked to colorectal cancer, facial dysmorphism, immunodeficiency, livedo, and short stature.

Immunogen

GST-tagged recombinant protein corresponding to human DNA polymerase II subunit A near the N-terminus.

Epitope: Near N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in human erythroleukemia K562 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DNA polymerase II subunit A in 10 µg of human erythroleukemia K562 cell lysate.

Specificity

Clone 9F11.1 reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. It does not recognize DNA polymerases alpha, beta or delta.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~260 kDa observed